Figure 4.

Compound ISOX and vorinostat increase MBNL1 expression in DM1-patient-derived fibroblasts. (A) Immunoblot images of MBNL1 levels in DM1 patient-derived (top) and normal (bottom) fibroblasts after 5 μM ISOX or 5 μM vorinostat treatment for 2 days. Three biological replicates were generated for each treatment. Tubulin was used as a loading control. (B) MBNL1 levels from A were normalized to tubulin signal and plotted as a bar graph. Error bars show the standard deviation (SD) of the biological triplicates and statistic differences were characterized by a t test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.