Figure 3.
(PR)50 and (GR)50 are localized to the nucleolus in C. elegans muscle cells (A) Imaging of live, anesthetized Day 1 adult C. elegans hermaphrodites expressing the indicated dipeptide. Green shows dipeptide-GFP localization expressed in muscle, red shows histone-tagRFP within the muscle nucleus. Arrowhead points to the histone-free nucleolar region. Scale bar=10 μm. (B) Quantification of DPR–GFP signal that is observed in nucleoplasm, nucleolus, and non-nuclear cellular compartments. N = 55–78 nuclei from five to six animals. Data shown are means ± S.D.