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. 2017 Sep 27;26(24):4916–4928. doi: 10.1093/hmg/ddx372

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Altering the rate of aging affects (PR)₅₀ but not (GR)₅₀ toxicity. (A) Paralysis assay of animals expressing GFP, (GR)₅₀ or (PR)₅₀ under the control of the myo-3 promoter in the wild-type or daf-2(e1370) background. ‘Day 0’ animals were isolated as L4 stage animals. N = 48–50 animals per genotype. n.s., ‘not significant’; **P < 0.01 versus GFP control (Log-rank test with Bonferroni adjusted P-value). (B) Paralysis assay of animals expressing (PR)₅₀ under the control of the myo-3 promoter in the wild-type, daf-16(mu86), or daf-2(e1370); daf-16(mu86) background. N = 48–50 animals per genotype. n.s., ‘not significant’; ***P < 0.001 versus (PR)₅₀ (Log-rank test with Bonferroni adjusted P-value). (C) Paralysis assay of animals expressing (PR)₅₀ under the control of the myo-3 promoter in the wild-type or hsf-1(sy441) mutant background. N = 48–50 animals per genotype. ***P < 0.001 versus wild-type (Log-rank test with Bonferroni adjusted P-value). (D) Fluorescent microscopy images of day 1 adult worms expressing either (GR)₅₀ or (PR)₅₀ (green) and soluble muscle mCherry (red) in the wild-type or daf-2(e1370) mutant background. Arrow points to site of nuclear DPR accumulation. Scale bar=10 μm. (E) Fluorescent microscopy images of Day 1 adult worms expressing (PR)₅₀ (green) and soluble muscle mCherry (red) in the daf-16(mu86) or daf-2(e1370); daf-16(mu86) mutant background. Arrow points to site of nuclear DPR accumulation. Scale bar=10 μm . (F) Fluorescent microscopy images of day 1 adult worms expressing (PR)₅₀ (green) and soluble muscle mCherry (red) in the wild-type or hsf-1(sy441) mutant background. Arrow points to site of nuclear DPR accumulation. Scale bar=10 μm.