Figure 3.

Structural analysis of the cloned NtMFP1 cDNAs. A, Schematic structure of the partial cDNAs isolated from a tobacco lambda ZAP cDNA library. T3, T1, and PCR#1 are shown as white boxes, represent overlapping fragments of the same gene (NtMFP1-1). T2 and T6 are shown as black boxes, represent overlapping fragments of a second gene (NtMFP1-2). The fragments derived from the NtMFP1 cDNA that were used as probes for Southern and RNA blots are indicated. B, Nucleotide sequence and deduced amino acid sequence of NtMFP1-1. Boxed regions indicate the two hydrophobic domains. Horizontal arrows indicate the oligonucleotide sequences used to amplify the PCR#1 fragment. Vertical arrows indicate the positions of intron sequences. The GenBank accession numbers for NtMFP1-1 and NtMFP1-2 are AF131231 and AF131232, respectively.