Figure 5.
ZC3H14 is required for proper poly(A) tail length control in mice. Bulk poly(A) tail length was analysed from brain (cortex and hippocampus) and liver tissue. (A) A representative gel of the resolved bulk poly(A) sample isolated from cortex is shown for Zc3h14Δex13/Δex13 (Δ13/Δ13) and Zc3h14+/+ (+/+) hippocampal samples isolated from three independent mice. Approximate positions of nucleotide size markers (nt) are indicated. (B) To provide a quantitative measure of poly(A) tail length across samples, the Relative Intensity of the signal is plotted along the length of the poly(A) tail as determined by the size markers. The positions of the scan corresponding to 200, 300, 400, and 600 nucleotides (nt) are indicated. Results are presented for Cortex (P = 0.11), Hippocampus (P < 0.0001) and Liver (P = 0.05).