Figure 7.
Validation of proteomic changes in Zc3h14+/+ compared to Zc3h14Δex13/Δex13 hippocampi. Total lysate was prepared from hippocampi of three Zc3h14+/+(+/+) and three Zc3h14Δex13/Δex13 (Δ13/Δ13) hippocampi. Samples were probed with antibodies to ZC3H14, Wwox, Cank2a, Sv2c, and a control protein Hsp90. A representative immunoblot is shown in (A) and the results from three independent samples are quantitated as a fold increase in (B). (C) To test whether ZC3H14 can bind to the CaMK2a transcript, we performed RNA immunoprecipitation from hippocampal lysates. Results are presented as fold enrichment compared to input for CaMK2a, a known target Atp5g1, and a control transcript Zc3h14.