Figure 5.

4PBA treatment ameliorated the tail shape (A) and normalized the neural spine slope (B) of Chi/+ fish after 14 weeks of treatment. Scale bar: 1 mm in (A) and 500 µm in (B). (C) Transmission electron microscopy of 11 dpf Chi/+ larvae showed a general reduction of the ER cisternae size after 4PBA treatment. Magnification 12000X. (D) Representative images of picro sirius red staining Chi/+ zebrafish regrown tail after 7 days from tail clipping in absence or presence of 4PBA. A strongest signal in the sample obtained from 4PBA treated fish confirmed a higher amount of collagen in the matrix following 4PBA treatment. White lines indicate the portion of tail regrown in 7 days. Magnification 10X.