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. 2017 Jun 29;26(19):3682–3698. doi: 10.1093/hmg/ddx252

Figure 7.

Figure 7

Schematic representation of the bioreactor. The bioreactor is composed of three scaffolds (6x3 mm, 400µm thickness, 0,6 µm fiber pore size), connected by microfluidic channels. PKH26+ FKRP L276IKI satellite cells were seeded on the first scaffold and secreted PKH26+ exosomes, able to reach not-infected SCs seeded on the second scaffold, running through a microfluidic channel connecting the two chambers (A). IF analysis of infected FKRP L276IKI SCs; GFP signal was detected in the cell cytoplasm (B). PKH26- labelled FKRP L276IKI SCs cells (C). PKH26- exosomes secreted by PKH26- labelled FKRP L276IKI SCs cells (D). Images were taken at 100X magnification. Immunofluorescent analysis of not-infected SCs seeded on the second scaffold. PKH26+ exosomes- (in red) targeted SCs recovered the expression of wild-type FKRP (in green). Nuclei were stained with Dapi (E, F).