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Rolling circle replication assay reveals dominant negative effect of POLγA:Y955C and POLγA:Y955H. (A) In vitro replication reactions performed at 10 µM dNTP and indicated POLγ versions (all experiments were performed in the presence of POLγB). Lanes 1–4, WT POLγA; lanes 5–8; POLγA:Y955H; lanes 9–12, WT POLγA and POLγA:Y955H; lanes 13–16, WT POLγA; lanes 17–20; POLγA:Y955C; lanes 21–24, WT and POLγA:Y955C. (B) As in (A) but at 1 µM dNTP.
