Figure 3.

Binding of FRMPD4^{p.Cys618ValfsX8} with known interacting proteins. (A) Domain structure of human FRMPD4 and its mutant protein. (B) Various FRMPD4 and Homer1c constructs were transfected into HEK293T cells. Anti-myc antibody immunoprecipitated FRMPD4-WT and FRMPD4^{p.Cys618ValfsX8}. Homer1C was co-immunoprecipitated by FRMPD4-WT, but not FRMPD4^{p.Cys618ValfsX8}. (C) Various FRMPD4 and PSD-95 constructs were transfected into HEK293T cells. Anti-myc antibody immunoprecipitated FRMPD4-WT and FRMPD4^{p.Cys618ValfsX8}. PSD-95 was co-immunoprecipitated by FRMPD4-WT while this binding was significantly reduced between FRMPD4^{p.Cys618ValfsX8} and PSD-95.