Figure 1.

Identification of novel antigens associated with in vitro plasticity of epithelial cells, identified with high-throughput surface profiling. (A) Schematic shows experimental workflow of high-throughput surface screen with fluorescently barcoded cell lines. Cell lines were harvested, barcoded with fluorescent amine-reactive dyes, pooled and stained in multiwell plates. After data acquisition, cell lines were deconvoluted based on their fluorescent barcode and processed to data analysis. (B) Antigens upregulated on the surface of epithelial cell lines. Antigens selected for further validation are highlighted in blue, pan-epithelial marker EpCAM is shown in green. (C) Antigens upregulated on the surface of mesenchymal cell lines. Antigens selected for further validation are highlighted in red.