Skip to main content
. 2017 Apr 25;26(14):2701–2718. doi: 10.1093/hmg/ddx158

Figure 3.

Figure 3

Altered morphology and distribution of microtubule, mitochondria, and acethylcholine receptor in mcoln1 zebrafish adult mutant. (A) Co-immunostaining for Cytochrome C, LC3, and DAPI in single muscle fiber dissected from 12-month-old WT and mcoln1ab−/− zebrafish. (B) Co-staining for actinin, α-Bungarotoxin, and DAPI in single muscle fiber dissected from 12-month-old WT and mcoln1ab−/− zebrafish. Arrows mark the acetylcholine receptor distribution in mcoln1ab−/− zebrafish muscle fiber. Scale bar, 10 μm. (C) Co-staining for LysoTracker and DAPI in live single muscle fiber isolated from 12-month-old WT and mcoln1ab−/− zebrafish. All the images are representative of at least three independent experiments. Scale bar, 50 μm.