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. 2017 Apr 25;26(14):2701–2718. doi: 10.1093/hmg/ddx158

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Published by Oxford University Press 2017. This work is written by US Government employees and is in the public domain in the US.

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Autophagy disruption in skeletal muscle of mcoln1ab^−/− fish (A–G) Muscle samples of 12-month-old mcoln1ab^−/− zebrafish were analyzed by transmission electron microscopy (TEM). Images show autophagic debris containing electro-dense inclusions, organelles and other cytoplasmic contents in mcoln1ab^−/− skeletal muscle. Arrows in (E) indicate multi-lamellar structures; arrowheads in (F) point to abnormal mitochondria; and the asterisks in (G) denotes autophagosomes. Scale bar for (A–F), 1 μm. Scale bar for (G), 250 nm.