Figure 1.

Levels of dimeric and oxidised DJ-1 are altered in human PD brain. (A) SDS PAGE and western blot analysis of human post mortem tissue lysates from 10 control individuals versus 10 PD patients. Membranes were probed for total DJ-1 (Enzo mouse monoclonal) to detect monomeric and dimeric forms of the protein. An antibody that detects oxidized DJ-1 at residue C106 (oxDJ-1) was also used. β-actin was used as a loading control. (B) Quantification of the western blots shown in (A). Statistical significance was calculated by one-way ANOVA with post hoc Tukey HSD test. *P < 0.05; ***P < 0.01 vs. control.