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. 2017 Jul 25;26(20):4028–4041. doi: 10.1093/hmg/ddx294

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Knock down of DJ-1 affects the dopaminergic and noradrenergic system. (A) Overview of catecholamine synthesis pathways. Abbreviations: tyrosine hydroxylase (TH), dopa decarboxylase (DDC), dopamine beta hydroxylase (DBH), phenylethanolamine-N-methyltransferase (PNMT). (B,C) Results of catecholamine ELISA to measure intracellular levels of dopamine (DA) and noradrenaline (NOR). Catecholamine levels were calculated as pg/mg protein. Results are expressed as percent of SH-SY5Y cells (n = 3). Statistical significance was determined by one-way ANOVA with post hoc Tukey HSD test. *P < 0.05 vs. SH; **P < 0.01 vs. SH; n.s., non-significant.