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cdc6tsu4305 mutant embryos undergo excess apoptosis. (A) Detection of active Caspase3. Embryos at 24 hpf were subjected to immunostaining with anti-active Caspase3 antibody and staining with DAPI and observed by confocal microscopy. (B) Detection of DNA fragmentation by TUNEL assay. All embryos were positioned with anterior to the left. (C) Detection of p-H2A.X in 24-hpf embryos by Western blotting. WT, wild-type siblings; MT, cdc6tsu4305 mutants.
