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. 2017 Dec 6;27(3):486–498. doi: 10.1093/hmg/ddx418

Figure 4.

Figure 4.

Apoptosis is increased in cardiac tissues of SMA mice. (A) TUNEL staining of cardiac tissues from both heterozygous (Het) and SMA mice aged P0, P2, P4, and P6. Compared with heterozygous mice, the number of TUNEL-positive SMA cardiomyocytes is markedly increased on P4 and P6. Nuclei were counterstained with DAPI. Scale bar, 20 μm. (B) Quantification of apoptotic cardiomyocytes identified by TUNEL staining, as shown in (A) (3 mice per group and 3 counts per mouse). ***P < 0.001. (C) TEM images of heterozygous (upper) and SMA (lower) cardiac tissues. Mitochondria are normal in heterozygous mice, but swollen in SMA cardiomyocytes. The arrowheads point to degenerating mitochondria with cristae deformation.