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. 2017 Dec 6;27(3):486–498. doi: 10.1093/hmg/ddx418

Figure 7.

Figure 7.

Effect of Survivin overexpression in SMN-depleted neonatal mouse cardiomyocytes. Cardiomyocytes isolated from P2 heterozygous mice were treated with two Smn1-specific siRNAs (siRNA-1 and siRNA-2), respectively, and a scrambled siRNA control. (A) Western blotting analysis showing that Survivin expression was markedly reduced in cardiomyocytes after knockdown of SMN with two independent siRNAs. Tubulin was used as a loading control. (B) Quantitation of SMN and Survivin protein levels after treatment with siRNA-1 or siRNA-2 in cardiomyocytes (n = 3). (C) Western blotting analysis showing proper expression of T7-tagged Survivin after transfection of the cDNA expression plasmid pCGT7-Survivin into SMN-depleted cardiomyocytes. Monoclonal anti-T7 antibody was used to detect T7-Survivin. (D) Quantitation of T7-Survivin levels after transfection of different amounts of pCGT7-Survivin. Tubulin was used as a loading control. (E–G) DNA profiles of cardiomyocytes transfected with control siRNA (E), or siRNA-2 with (G) or without (F) overexpression of Survivin (0.6 μg plasmid). Plasmid pCGT7-Survivin was transfected into cells 24 h post siRNA-2 transfection; 48 h later, cells were harvested for analysis (n = 3). (H) Histogram of cell-cycle data from (E–G). *P < 0.05 **P < 0.01.