Skip to main content
. 2017 May 3;26(14):2634–2648. doi: 10.1093/hmg/ddx147

Figure 5.

Figure 5

Rescue of DISC1-TR induced neurodevelopmental and neurofunctional phenotypes via DISC1-TR regulated effector expression.(A, B) Representative traces and group data showed that expression of Dys (elav/+; DISC1-TR/UAS-DysDp186) successfully restored both the increased EJC amplitude and reduced PPF ratio induced by expression of DISC1-TR (elav/+; DISC1-TR/+) (n = 16). Expression of the L(C) isoform of Shot (elav/+; DISC1-TR/UAS-Shot.L(C)29042) only rescued increased EJC amplitude phenotype of DISC1-TR expressed flies (elav/+; DISC1-TR/+) (n = 16). The groups that showed rescue are marked with #, which indicates a statistical significance (P < 0.05, post-hoc test) with DISC1-TR expression group (elav/+; DISC1-TR/+) and statistical no significance (P > 0.05, post-hoc test) with control group (elav/+) after ANOVA. (C, D) Statistical analysis of properties of NMJ boutons morphology. Only Trio overexpression (elav/+; DISC1-TR/UAS-Trio9513) succeeded in restoring the number of Is boutons (n = 12), while larvae with overexpression of the L(C) isoform of Shot (elav/+; DISC1-TR/UAS-Shot.L(C)29042) rescued satellite bouton number (n = 14). The statistical analysis is the same as that in figure A. (E) Flies co-expressing Dys with DISC1-TR (elav/+; DISC1-TR/UAS-DysDp186) exhibited same learning performance as controls (elav/+) and the DISC1-TR group (elav/+;DISC1-TR/+). However, the expression of Trio (elav/+; DISC1-TR/UAS-Trio9513) and both isoforms of Shot (elav/+; DISC1-TR/UAS-Shot.L(A)29044 and elav/+; DISC1-TR/UAS-Shot.L(C)29042) showed decreased learning performance compare to those two groups (ANOVA, n =  6-8). The reversal learning performance was further reduced in Trio (elav/+; DISC1-TR/UAS-Trio9513) and both isoforms of Shot (elav/+; DISC1-TR/UAS-Shot.L(A)29044 and elav/+; DISC1-TR/UAS-Shot.L(C)29042) expressing flies compared to that of the DISC1-TR expressing flies, while the Dys co-expressing flies (elav/+; DISC1-TR/UAS-DysDp186) showed the same reversal learning ability as the DISC1-TR expressing flies, which was lower than that of the control (elav/+) (ANOVA, n = 6). All data are represented as mean ± SEM.