Figure 2.

ALS mutant SOD1 G93A does not affect resting [Ca²⁺]_c. Cortical neurons were transduced with EGFP, EGFP-SOD1 WT or G93A lentivirus and [Ca²⁺]_c determined by of Fura2 ratio imaging. To ensure that only viable neurons were taken into account, a transient Ca²⁺ influx was invoked by depolarization with 50 mM KCl. Average Ca²⁺ traces are shown in (A). Resting [Ca²⁺]_c was calculated as the average [Ca²⁺]_c between 50 and 150 s of recording and values for individual cells averaged to generate the bar graphs in (B). SOD1 G93A did not change resting [Ca²⁺]_c in comparison to EGFP control or SOD1 WT-expressing neurons. Results are shown as mean ± SEM, statistical significance was determined by one-way ANOVA, N (cells) = EGFP: 32, WT: 36, G93A: 37 from 3 experiments.