Figure 4.

Poly GA is most toxic DPR in chick spinal cord. (A) Transverse sections of embryonic day 3.5 (E3.5) chick embryo spinal cords electroporated with DPR constructs as labelled (GA, GP, GR, PA and PR) with 4 µg of plasmid, which causes toxicity in the chick spinal cord. (a–e) Expression of DPR detected with GFP Immunostaining on sections of electroporated spinal cord. (f–j) Cell toxicity of DPRs were assessed by TUNEL assay. (k–o) Merge image of DAPI and DPR. Scale bar = 100 μm. (B) Quantitative analysis of TUNEL-positive cells showing that cells bearing inclusions of GA, PR have significantly increased TUNEL positive staining (***P<0.0001).