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. 2017 Aug 21;26(22):4395–4405. doi: 10.1093/hmg/ddx325

Figure 1.

Figure 1.

Phenotype analysis of 6-week-old wild-type and rAAV-treated G6pt−/− mice. (A) Liver microsomal G6P uptake activity and hG6PT mRNA expression. The data were obtained from wild-type (+/+, n = 8), GPE (n = 12) and miGT (n = 12) mice. (B) Blood glucose levels. (C) BW, LW, and LW/BW of mice. The data were obtained from wild-type (+/+, n = 24), GPE (n = 13) and miGT (n = 15) mice. (D) Blood neutrophils counts expressed as % of white blood cells. The data were obtained from wild-type (+/+, n = 16), GPE (n = 6) and miGT (n = 7) mice. (E) Bone marrow neutrophil respiratory burst activity in response to 200 ng/mL of PMA and calcium flux activity in response to 10−6 M of fMLP. Wild-type (○, n = 3); GPE/miGT (•). The data from GPE (n = 2) and miGT (n = 2) were indistinguishable and grouped together. (F) Antibodies against hG6PT. Microsomal proteins from Ad-hG6PT (20) infected COS-1 cells were electrophoresed through a single 12% polyacrylamide-SDS gel and transferred onto a PVDF membrane. Membrane strips, representing individual lanes on the gel were individually incubated with the appropriate mouse serum at 1: 50 dilution. A polyclonal anti-hG6PT antibody (20) was used as a positive control. Lanes 1, 2: anti-hG6PT antiserum; lanes 3, 5, 7, 9, 11, 13: serum samples from wild-type mice; serum samples from GPE (lanes 4, 6, 8) or miGT (lanes 10, 12, 14) mice. Data represent the mean ± SEM. *P < 0.05, **P < 0.005.