Table 1.

Clinical information on samples assigned to methylation cluster 2 compared with methylation cluster 3 and samples assigned to EOPE methylation subcluster 1 and EOPE methylation subcluster 2. *p-value<0.1, **p-value<0.05, ***p-value<0.01.

DISCOVERY COHORT
	Methylation Cluster 2	Methylation Cluster 3	EOPE Methylation Subcluster 1	EOPE Methylation Subcluster 2
N =	43	27	8	16
IUGR Status (IUGR/Total)	10/43 (23%)*	2/27 (7%)	7/8 (88%)	14/16 (88%)
Fetal Sex (F/Total)	22/43 (51%)	11/27 (41%)	5/8 (63%)	3/16 (19%)*
Gestational Age (weeks) [range (mean)]	25.0–40.0 (36.0)	28.0–41.3 (35.7)	24.9–36.0 (31.7)	26.0-37.3 (32.6)
Fetal Birth Weight (SD) [range (mean)]	−2.78–3.77 (−0.44)**	−2.16–1.70 (0.18)	−2.90–1.17 (−1.87)	−8.19 - -0.58 (-2.26)
Chronic Hypertension (CH/Total)	7/43 (16%)	2/27 (7%)	2/8 (25%)	5/16 (31%)
Diabetes (Pre-existing or Gestational) (Diabetes/Total)	2/43 (5%)	0/27 (0%)	1/8 (13%)	1/16 (6%)
Chorioamnionitis (CA/Total)	7/43 (16%)	5/27 (19%)	0/8 (0%)	0/16 (0%)
Premature Rupture of Membranes (PPROM/Total)	3/43 (7%)	4/27 (15%)	0/8 (0%)	0/16 (0%)
Ultrasound Findings (Findings/Total)	7/43 (16%)	2/27 (7%)	3/8 (38%)	4/16 (25%)
Placental Pathology Noted (Notes/Total)	17/43 (40%)	7/27 (26%)	4/8 (50%)	10/16 (63%)
VALIDATION COHORT
	Methylation Cluster 2	Methylation Cluster 3	EOPE Methylation Subcluster 1	EOPE Methylation Subcluster 2
N =	7	11	19	11
IUGR Status (IUGR/Total)	0/7 (0%)	0/11 (0%)	11/19 (58%)	7/11 (64%)
Fetal Sex (F/Total)	1/7 (14%)	5/11 (45%)	9/19 (47%)	7/11 (64%)
Gestational Age(weeks) [range (mean)]	30.0–37.0 (33.0)***	37.0–40.0 (38.4)	27.0–37.0 (32.8)***	26.0-34.0 (29.8)
Fetal Birth Weight (SD) [range (mean)]	−0.48–0.50 (−0.06)	−1.11–3.60 (0.34)	−2.27–2.92 (−1.12)	−2.46 - -0.86 (-1.59)
Chronic Hypertension (CH/Total)	1/7 (14%)	1/11 (9%)	7/19 (37%)	3/11 (27%)