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. 2017 May 31;26(16):3116–3129. doi: 10.1093/hmg/ddx196

Figure 2.

Figure 2

Determination of the amino acids inserted during G418-mediated suppression of UGA, UAA and UAG codons in the same context in HEK293 cells. (A) PTCs and context used to determine amino acids inserted (and their relative proportions). (B) Representative western blot showing full-length proteins produced after 24 h of G418 treatment in HEK293 cells transiently transfected with the QXQ constructs. (C) Comparison of amino acids inserted (and relative frequencies of each) as determined from by MS–MS of samples purified from HEK293 cells. Raw spectra of the QXQ constructs can be seen in Supplementary Material, Figure S1.