Figure 3.

Rapamycin does not improve TK2 mouse fitness. (A) Pup weight of rapamycin-treated and untreated litters was measured daily, and indicated for untreated WT (black filled squares), rapamycin-treated WT (black open squares), untreated TK2 (gray filled squares), and rapamycin-treated TK2 (gray open squares) mice. Error bars represent standard deviation, when applicable. (B) Representative image of WT and TK2-treated and untreated mice at 15 days, with weights indicated. (C) Motor function was assessed using a rotating rod assay by recording length of time mice could remain on the rod, up to 30 s. Average values are shown, with error bars indicating standard deviation and asterisk indicating significance compared with untreated WT at the 95% confidence interval by Welch’s P-value; n = 7 for untreated WT and TK2 groups, n = 9 for rapamycin-treated WT and TK2 mice. Other notation as in Figure 2.