Table 1.
Univariate analysis of liver and plasma metabolites in rapamycin-treated vs untreated mice
| Liver | |||
|---|---|---|---|
| Metabolite | WT ± rapa(Fold change) | TK2 ± rapa(Fold change) | Pathway |
| L-glutamine | 1.3 | Amino acid metabolism | |
| L-methionine | 1.5 | ||
| N-acetyl-l-alanine | 1.3 | ||
| 5-oxo-l-proline | 1.5 | 1.6 | |
| Trans-4-hydroxyproline (∗) | 1.2 | 1.6 | |
| L-glutamic acid | 1.5 | 1.5 | |
| 4-acetamidobutanoic acid (∗) | 1.4 | 1.4 | |
| Ketoleucine | 1.7 | 1.4 | |
| 2-hydroxyglutarate | 1.4 | 1.4 | |
| Taurine | 0.7 | 0.8 | |
| S-(5'-adenosyl)-l-homocysteine | 0.6 | 0.6 | |
| L-pipecolic acid (∗) | 0.6 | 0.4 | |
| Guanidinoacetate | 0.6 | ||
| Hypotaurine | 0.3 | ||
| N-acetyl-d-galactosamine | 1.4 | 1.9 | Carbohydrate metabolism |
| Melibiose | 0.4 | ||
| D-(+)-raffinose | 0.4 | ||
| Palatinose | 0.4 | ||
| D-ribose | 0.6 | 0.6 | |
| Nicotinamide | 0.7 | Cofactor metabolism | |
| 6-carboxyhexanoate | 1.5 | ||
| 4-pyridoxate | 0.6 | ||
| 3-(4-hydroxyphenyl)lactate | 0.7 | ||
| Omega-hydroxydodecanoic acid | 1.5 | Fatty acid/lipid metabolism | |
| Ethylmalonic acid | 0.9 | 0.6 | |
| Glyoxylic acid | 1.6 | ||
| Myo-inositol | 0.8 | ||
| N-methyl-l-glutamate | 0.5 | Methane metabolism | |
| Uridine | 1.4 | Nucleotide metabolism | |
| Deoxycytidine | 2.3 | ||
| 5'-methylthioadenosine | 2.0 | ||
| Cytidine | 1.6 | ||
| Uracil | 0.7 | 0.7 | |
| Xanthine | 0.7 | ||
| Inosine | 0.7 | ||
| Adenosine | 0.6 | ||
| 2-hydroxybutyric acid | 0.8 | Oxidative stress | |
| Alpha-ketoglutaric acid | 2.0 | 2.0 | TCA cycle |
| L-fucose | 0.7 | 0.7 | |
| Plasma | |||
| Alpha-ketobutyrate | 1.4 | Amino acid metabolism | |
| N-acetylserine | 1.3 | ||
| 4-acetamidobutanoic acid (∗) | 1.5 | 1.7 | |
| Trans-4-hydroxyproline (∗) | 1.3 | 1.6 | |
| N-acetyl-l-alanine | 1.3 | 1.4 | |
| O-acetyl-l-serine | 1.3 | 1.3 | |
| L-pipecolic acid (∗) | 0.7 | 0.7 | |
| Ketoleucine | 0.8 | ||
| L-lysine | 0.7 | ||
| L-tryptophan | 0.6 | ||
| Phenylpyruvate | 0.3 | ||
| Propanoate | 0.8 | 0.8 | β-oxidation |
| Gluconic acid | 1.3 | Carbohydrate metabolism | |
| N-acetylneuraminic acid | 1.6 | 1.7 | |
| L-arabitol | 1.3 | 1.3 | |
| 4-hydroxybenzoate | 0.7 | Cofactor metabolism | |
| Pyridoxal | 0.7 | 0.7 | |
| β-Glycerophosphoric acid | 0.6 | Glycerolipid metabolism | |
| Creatinine | 1.2 | Muscle breakdown | |
| 2-amino-2-methylpropanoate | 1.4 | Nucleotide metabolism | |
| Inosine | 0.6 | ||
| Xanthine | 0.7 | ||
| Ophthalmic acid | 4.2 | Oxidative stress | |
*
Numbers indicate fold change of significantly altered metabolites (p>0.05); upregulated values (treated/untreated) shown in red, downregulated in blue. Metabolites are also shown as colored data points in Supplementary Material, Fig. S9. Blank boxes denote no significant change. Compounds highlighted in bold were altered significantly in both the WT±rapa and TK2±rapa columns. Asterisks indicate the compounds identified in both liver and plasma.