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. 2017 Oct 12;27(1):14–29. doi: 10.1093/hmg/ddx380

Figure 6.

Figure 6.

Klotho increases satellite cell proliferation through a TNFα-mediated mechanism. (A) Myoblast cell numbers per well after growth in culture media only (Control; white bar) or conditioned media from wild-type BMDMs, Klotho transgenic BMDMs (KL Tg) or Klotho hypomorphic mutants (KL mutants). For each sample group grown with conditioned media, 5 wells contained conditioned media only (grey bars), 5 wells contained conditioned media to which isotype control IgG had been added (black bars) and 5 wells contained conditioned media with anti-Klotho (striped bars). * indicates significant difference from control cultures at P < 0.05. # indicates significant difference from isotype control cultures from same conditioned media culture group at P < 0.05. (B) Myoblast cell numbers per well after growth in culture media only or culture media containing recombinant Klotho (KL treated). For each of those treatment groups, 5 wells contained no antibody (white bars), 5 wells contained isotype control antibody (grey bars) and 5 wells contained anti-TNFα (black bars). * indicates significant difference from control cultures at P < 0.05. # indicates significant difference from isotype control cultures at P < 0.05. (C) Protein concentration in isovolumetric homogenates of myotubes grown and stimulated under same conditions as myoblasts in Figure 6C. * indicates significant difference from control cultures at P < 0.05.