Figure 2.

A subset of pairwise combinations of 16p11.2 homologs alter brain and ventricle morphology. (A) Definition of an interacting pair. Dorsal views of 24 hpf embryos with red dye injected into the ventricles. Single gene LOFs were induced to about ∼50% normal RNA remaining (or a sub-phenotypic dose) and generally resembled control MO-injected embryos. If a pair of genes interacted, then suppressing them together resulted in abnormal brain and ventricle morphology. (B) Pie chart of all 162 pairwise combinations tested. (C) Map of interacting pairs of genes, with the lines drawn between two genes if they interact, and scaling the font size of the gene name according to the number of interactions it has.