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. 2017 Nov 3;27(1):160–177. doi: 10.1093/hmg/ddx397

Figure 6.

Figure 6.

CHCHD10 expression in mouse tissues. (A) Representative Western blot using antibodies against CHCHD10, CHCHD2, and Tim23 (as mitochondrial loading control), in crude mitochondrial extracts prepared from WT mouse tissues. 50 μg of protein was loaded per sample. SC = spinal cord. (B) Input, flow through, and eluate fractions from CHCHD10, CHCHD2, and IgG control Co-IP in heart mitochondria. Immunoblot: CHCHD10, CHCHD2, p32, mitofilin. (C) CHCHD10 immunoreactivity in brain section containing substantia nigra (SN) region from WT mouse. PAG = periaqueductal gray, IPN = interpeduncular nucleus. Left panel: bar = 50 μm. Right panel: bar = 10 μm. (D–E) Immunohistochemistry of brain sections stained with CHCHD10 and TH antibodies. Bar = 100 µm in D, bar = 10 µm in E. (F–G). Immunohistochemistry of spinal cord sections stained with CHCHD10 and ChAT antibodies. Ba r = 100 µm in F, bar = 30 µm in G.