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. 2017 May 10;26(15):3014–3027. doi: 10.1093/hmg/ddx188

Figure 4.

Figure 4

Targeted sodium bisulfite pyrosequencing of cg05575921 and cg07992500 in CSC-treated A549 cells. A549 cells were exposed in quadruplicate to CSC in vitro for 48 h and 2 weeks at 0, 5, 10 or 20 µg/ml. DNA methylation was assessed by pyrosequencing of sodium bisulfite-treated DNA. All 4 plots showed a significant trend as indicated in the chart and a statistically significant reduction in DNA methylation between 0 and 20 µg/ml CSC (AHRR: P = 0.009 and 0.0177 at 48 h and 2 weeks respectively; CYP1B1: P = 0.0196 and 0.0297 at 48 h and 2 weeks, respectively (t-test, one-sided)).