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. 2017 May 29;26(17):3235–3252. doi: 10.1093/hmg/ddx206

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Figure 3. — Shortened poly(A) tails in Pabpn1^+/A17 mice. (A) Poly(A) tail length was analyzed by polyacrylamide gel electrophoresis of 3’ end labeled poly(A) tracts from total RNA isolated from rectus femoris muscles of three-month-old male Pabpn1^+/+ and Pabpn1^+/A17 mice. n = 3 per genotype. *P < 0.05 (B) Quantification of regions representing short (1), medium (2), and long (3) poly(A) tails noted in (A) normalized to the total short poly(A) tails marked in (A) (Normalize) revealed fewer long poly(A) tails in Pabpn1^+/A17mice. (C) Quantification of gel from (A) by plotting density along each lane revealed shorter poly(A) tails in Pabpn1^+/A17mice.