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. Author manuscript; available in PMC: 2018 Apr 5.
Published in final edited form as: J Immunol. 2017 Jan 23;198(5):1815–1822. doi: 10.4049/jimmunol.1601434

Figure 3. Basophil and mast cell sensitisation in Il4-hemizygous mice.

Figure 3

Basophils and mast cells were isolated from the immunised Basoph8 mice from Figure 2. A & C show representative flow analysis of basophils, B & D respectively show the geometric mean fluorescence intensity (gMFI) of IgE and NP-APC on both basophils and mast cells. C57BL/6J IL-4+/+ and IL-4G4/+ mice were immunised i.d. in the ear pinnae with NP-OVA/Alum and the gMFI of FceR1α on splenic basophils (E) and peritoneal mast cells (F) measured 7 days later. Data points show individual mice combined from two similar experiments (n=3-5 mice/group) in B and D and a single experiment (n=2-3 mice/group) in E and F, while lines show geometric means. Numbers on FACS histograms indicate sample gMFI. *p≤0.05 two-tailed Student's t-test after log transformation.