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. Author manuscript; available in PMC: 2018 Apr 5.
Published in final edited form as: J Immunol. 2017 Jan 23;198(5):1815–1822. doi: 10.4049/jimmunol.1601434

Figure 7. Effect of Il4-hemizygosity on CD4+ T cell IL-4 production.

Figure 7

Seven days after i.d. ear immunisation of C57BL/6J background mice with NP-OVA/Alum (A), or of BALB/c-ByJ background mice with OVA/alum (B), auricular lymph node CD4+ T cells were purified by positive selection and restimulated on anti-CD3-coated 96-well plates for IL-4 ELISA analysis. Data points show responses from individual mice from a single experiment (n=3 mice/group) in A and pooled from three experiments (n=2-3 mice/group per experiment) in B. ***p<0.01 by One-way ANOVA with Tukey's post-test.