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. 2018 Apr 5;13(4):e0195304. doi: 10.1371/journal.pone.0195304

Fig 5. Spatiotemporal expression of Col2a1 in the PS cartilage pads and IpL osteoligamentous junction during pregnancy and the postpartum period.

Fig 5

(A- F) Distribution of Col2a1 mRNA expression in hyaline cartilage (HC), near the subchondral bone (SB), in fibrocartilage (FC) at NP and 1dpp PS, and in BDR and BPR regions of the osteoligamentous junction from D19 to 5dpp. Col2a1-positive cells exhibit variable phenotypes: round cells at SB, elongated cells at the BPR, angular chondrocyte-like cells (HC/BDR) and elliptical chondrocyte-like cells (FC) from NP to 10dpp PS (arrowheads). (G-L) Areas temporally immunostaining to procollagen encoded by Col2a1. Positive cells presented a coincident phenotype and localization with Col2a1 mRNA-positive cells (arrowheads) (1:2000 anti-DIG pod/H-O). In situ hybridization (ISH) and Immunohistochemistry (IHC) experiments. Scale bars = 20 μm.