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. 2018 Apr 5;13(4):e0195304. doi: 10.1371/journal.pone.0195304

Fig 7. Spatiotemporal expression of Runx2 in the PS cartilage pads and IpL osteoligamentous junction during pregnancy and the postpartum period.

Fig 7

(A, F, G and L) Runx2 mRNA and protein were localized in round cells at the subchondral bone (SB) and hyaline cartilage (HC) cells with faded staining in NP and 10dpp PS (arrowheads). (B, C, E, H, I and K) At the IpL osteoligamentous junction, Runx2 mRNA expression and protein were observed primarily in elongated or round cells at the BPR region at D19, 1dpp and 5dpp (arrowheads), (D and J) while at 3dpp, faded staining for mRNA and protein were observed in elongated cells of both the BPR and BDR (arrowheads) regions (1:2000 anti-DIG pod/H-O). In situ hybridization (ISH) and Immunohistochemistry (IHC) experiments. (A-F) Scale bars = 20 μm. (G-L) Scale bars = 30 μm.