Figure 3.

Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted mRNA expressions were analyzed by reverse transcription and quantitative real-time PCR. (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). *P < 0.05 vs. untreated (Ut) and 0.