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The effect of glucose/glucose oxidase (G/GO) treatment on the DNA binding activities of activator protein-1 (AP-1), NF-κB, and STAT3 in 3T3-L1 adipocytes. Cells were treated with G/GO for the indicated time periods, and then nuclear proteins were isolated. DNA binding activities of (A) AP-1, (B) NF-κB, and (C) STAT3 were determined using electrophoretic mobility shift assay.
