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. 2018 Mar 26;14(3):e1007305. doi: 10.1371/journal.pgen.1007305

Fig 6. EGL-6 functions in URX neurons to regulate oxygen-dependent fat loss.

Fig 6

(A) Worms from a transgenic line bearing egl-6 expression using the flp-8 promoter were subjected to the oxygen-dependent fat loss assay. Non-transgenic animals (-) and transgenic animals (+) are shown. Relative to non-transgenic controls (light gray bars), transgenic animals (dark gray bars) restore body fat content at 10% (low) oxygen to that seen in wild-type animals. Data are expressed as a percentage of body fat in wild-type animals + SEM (n = 16–20). NS, not significant and *, p<0.05 by one-way ANOVA. (B) Representative fluorescent images showing the expression of Pegl-6::GFP (left panels), Pflp-8::mCherry (central panels), and their co-localization in URX (merge, right panels). egl-6 expression was also observed in DVA, SDQL, SDQR, HSN (not depicted) and additional head neurons. (C) The indicated genotypes were subjected to the oxygen-dependent fat loss assay. Fat content was quantified for each genotype and condition. White bars, fed; gray bars, fasted at 21% oxygen and black bars, fasted at 10% oxygen. Data are expressed as a percentage of body fat in wild-type fed controls + SEM (n = 16–20). NS, not significant and *, p<0.05 by one-way ANOVA.