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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Eur J Immunol. 2017 Aug 28;47(12):2059–2069. doi: 10.1002/eji.201747091

Figure 1.

Figure 1

IL-1β abrogates CD4+ T cell help for a protective IFN-γ secreting CD8+ T cell response. Mice were immunized with P18-I10 (CD8 epitope) or PCLUS3-18IIIB (CD4 helper + CD8 epitope), 7 days later, spleens were removed and expression of P18-I10 tetramer, and CXCR3 and T-bet on live tetramer specific CD3+CD8+ T cells was analyzed (A, C, D). Splenocytes were stimulated in vitro with P18-I10 peptide overnight and expression of IFN-γ was measured in CD8+ T cells (B). 10 days post-vaccination, mice were infected i.p. with 2 × 107pfu of vPE16. 5 days later viral pfu was determined in the ovaries (E). Each experiment was performed three times. Mann whitney test was used to determine significance. * indicates P<0.05.