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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Eur J Immunol. 2017 Aug 28;47(12):2059–2069. doi: 10.1002/eji.201747091

Figure 3.

Figure 3

T helper 17 cells provide help for Tc17 response but not a Tc1 response. A-C. negatively selected purified CD8+ T cells (10̂6) were cocultured with syngeneic DCs (2×10̂5), and 10̂6 polarized CD4+ Th1 or Th17 cells prepared as described in Materials and Methods, along with anti-CD3 antibody in solution. A. BALB/c (left column) and C57BL.6 (right column)CD8+ T cells receiving help from Th1 cells (middle row) or Th17 cells (bottom row) were analyzed for IL-17 and IFN-γ production. B. Multiple similar experiments in BALB/c mice were compared to show statistics. C. In a similar culture experiment, the cells were permeabilized and stained for intracellular T-bet and RORγt, transcription factors for IFN-γ and IL-17, respectively. D. In a similar culture, the CD8+ T cells cultured with Th1, Th17 or no Th were stained for granzyme B expression. Each experiment was performed three times. Wilcoxon rank test was used to determine significance. * P<0.05, and **P<0.01.