Figure 6.

Blockade of TGF-β inhibits induction of Th17 and Tc17 response by IL-1β and restores protective Tc1 response. Mice were administered intraperitoneally an anti-TGF-β antibody one day prior to being immunized with P18-I10 (CD8 epitope) or PCLUS3-18IIIB (CD4 helper + CD8 epitope) and were given this antibody every other day. Seven days post-immunization, spleens were removed and stimulated in vitro with P18-I10 peptide or PCLUS3-18IIIB peptide overnight and expression of IL-2 and IL-17A was measured in CD4⁺ T cells (A)and IFN-γ and IL-17A was measured in CD8⁺ T cells (B). % of CD8⁺ T cells that were positive for P18-I10-H2Dd tetramers were also stained for each group in the spleen (C) and lymph nodes (D). 10 days post-vaccination, mice were infected i.p. with 2 × 10⁷pfu of vPE16. 5 days later viral pfu were determined in the ovaries (E). Mann Whitney test was used to determine significance between groups of mice. Data shown are representative of two independent experiments. * P<0.05.