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. Author manuscript; available in PMC: 2018 Jul 21.
Published in final edited form as: Circ Res. 2017 May 18;121(3):244–257. doi: 10.1161/CIRCRESAHA.116.310308

Figure 2. FPR2/ALX knockdown does not affect barrier protective effect of OxPAPC against thrombin-induced permeability.

Figure 2

Pulmonary EC transfected with non-specific or FPR1, FPR2/ALX, or FPR3 specific siRNAs were treated with thrombin (0.5 U/ml), with or without OxPAPC pretreatment (15 μg/ml, 30 min). A - Analysis of EC permeability for macromolecules using FITC-labeled avidin as a tracer; n=6, *P < 0.01 vs. thrombin alone. B - MLC di-phosphorylation was evaluated using phospho-MLC specific antibody in control, thrombin, or OxPAPC treated EC. C - Effects of OxPAPC and LXA4 (100 nM) on thrombin-induced MLC phosphorylation were evaluated by Western blotting. Probing for β-tubulin was used as a normalization control. Numerical data depict results of quantitative densitometry; n=4; p<0.05 vs. thrombin alone.