Figure 2. Gain-of-function mutant of the JAK kinase hop significantly rescues GSC loss but not centrosome/spindle orientation caused by dome^RNAi.

(A–B) Examples of apical tip in nos-gal4 > UAS-dome^RNAi (A) and hop^tum-1, nos-gal4 > UAS- dome^RNAi (B) testes. Double-headed arrows indicate the gap between the hub and nearest germ cells, showing GSC loss. Green: Vasa. Red: FasIII. Blue: DAPI. Bar: 5 μm. (C) GSC numbers in the indicated genotypes. P value was calculated using two-tailed Student’s t-test. Error bars indicate the standard deviation. (D–E) Examples of spindles in control (D) and hop^tum-1, nos-gal4 > UAS-dome^RNAi (E) GSCs (indicated by a white dotted line). Asterisk indicates the hub. Arrowheads indicate spindle poles. Green: Vasa. Red: Fas III and γ-Tubulin. White: PH3. Blue: DAPI. Bar: 5 µm. (F) Summary of GSC centrosome/spindle misorientation in the indicated genotypes. P value comparing to control was calculated using two-tailed Student’s t-test. Error bars indicate the standard deviation. N = GSC number scored for centrosome orientation or mitotic GSC number scored for spindle orientation.