Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 26;7:e33685. doi: 10.7554/eLife.33685

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018, Chen et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 5. — (A) Co-immunoprecipitation of Dome with Eb1. Dome-GFP was pulled down from GSC extracts using an anti-GFP antibody and blotted using anti-Eb1 and anti-GFP. (B–C) Examples of centrosomes in control (B) and nos-gal4 > UAS-eb1^RNAi (C) GSCs (outlined by a dotted line). Asterisk indicates the hub. Arrowheads indicate centrosomes. Green: Vasa. Red: Fas III and γ-Tubulin. Blue: DAPI. Bar: 5 µm. (D–E) Examples of mitotic spindles in control (D) and nos-gal4 > UAS-eb1^RNAi (E) GSCs (outlined by a dotted line). Asterisk indicates the hub. Arrowheads indicate spindle poles. Green: Vasa. Red: Fas III and γ-Tubulin. White: PH3. Blue: DAPI. Bar: 5 µm. (F) GSC spindle orientation in the indicated genotypes. P value was calculated using two-tailed Student’s t-test. Error bars indicate the standard deviation. N = mitotic GSCs scored. (G–J) Eb1 localization in interphase control GSC (G), nos-gal4ΔVP16, gal80^ts > UAS-dome^RNAi (4 d after RNAi induction) GSC (H), control GB (I), and nos-gal4ΔVP16, gal80^ts > UAS-dome^RNAi (4 d after RNAi induction) GB (J). Asterisk indicates the hub. Green: α-Tubulin. Red: Eb1. Bar: 5 µm. (K–N) Eb1 localization in metaphase control GSC (K), nos-gal4ΔVP16, gal80^ts > UAS-dome^RNAi (4 d after RNAi induction) GSC (L), control GB (M), and nos-gal4ΔVP16, gal80^ts > UAS dome^RNAi (4 d after RNAi induction) GB (N). Asterisk indicates the hub. Green: α-Tubulin. Red: Eb1. Bar: 5 µm. (O–P) Pixel intensity analyses of Eb1 at interphase (O) and mitotic (P) GSCs in control and nos-gal4ΔVP16, gal80^ts > UAS-dome^RNAi (4 d after RNAi induction) testes. P value was calculated using two-tailed Student’s t-test. Error bars indicate the standard deviation.

Figure 5—figure supplement 1. — (A) Co-immunoprecipitation of Dome with Eb1. Dome-GFP was pulled down from GSC extracts using an anti-GFP antibody and blotted using anti-Eb1 and anti-GFP. (B–C) Examples of centrosomes in control (B) and nos-gal4 > UAS-eb1^RNAi (C) GSCs (outlined by a dotted line). Asterisk indicates the hub. Arrowheads indicate centrosomes. Green: Vasa. Red: Fas III and γ-Tubulin. Blue: DAPI. Bar: 5 µm. (D–E) Examples of mitotic spindles in control (D) and nos-gal4 > UAS-eb1^RNAi (E) GSCs (outlined by a dotted line). Asterisk indicates the hub. Arrowheads indicate spindle poles. Green: Vasa. Red: Fas III and γ-Tubulin. White: PH3. Blue: DAPI. Bar: 5 µm. (F) GSC spindle orientation in the indicated genotypes. P value was calculated using two-tailed Student’s t-test. Error bars indicate the standard deviation. N = mitotic GSCs scored. (G–J) Eb1 localization in interphase control GSC (G), nos-gal4ΔVP16, gal80^ts > UAS-dome^RNAi (4 d after RNAi induction) GSC (H), control GB (I), and nos-gal4ΔVP16, gal80^ts > UAS-dome^RNAi (4 d after RNAi induction) GB (J). Asterisk indicates the hub. Green: α-Tubulin. Red: Eb1. Bar: 5 µm. (K–N) Eb1 localization in metaphase control GSC (K), nos-gal4ΔVP16, gal80^ts > UAS-dome^RNAi (4 d after RNAi induction) GSC (L), control GB (M), and nos-gal4ΔVP16, gal80^ts > UAS dome^RNAi (4 d after RNAi induction) GB (N). Asterisk indicates the hub. Green: α-Tubulin. Red: Eb1. Bar: 5 µm. (O–P) Pixel intensity analyses of Eb1 at interphase (O) and mitotic (P) GSCs in control and nos-gal4ΔVP16, gal80^ts > UAS-dome^RNAi (4 d after RNAi induction) testes. P value was calculated using two-tailed Student’s t-test. Error bars indicate the standard deviation.