Extended Data Table 2.

Human IR ECD binding and kinetic data. Top: Human IR ECD binding data for recombinant human insulin. Data is reported as the geometric mean for 23 replicates; the geometric standard deviation is shown in parentheses. The potency measured with the ¹²⁵I-insulin competition assay agrees very well with values from the literature¹⁷.

Bottom: Human IR ECD Binding Kinetic Data for RHI. Data is reported as the geometric mean for three replicates; the geometric standard deviation is shown in parentheses. The SPR data is very consistent with values from the literature³¹.

[125I]-Insulin Competition Binding: IC50 (nM)	Biacore Binding: High Affinity Site Kd (nM)	Biacore Binding: Low Affinity Site Kd (nM)
3.0	2.7	220
(2.6)	(1.2)	(1.7)

Biacore Binding: High Affinity Site kon (nM−1 s−1)	Biacore Binding: High Affinity Site koff (s−1)	Biacore Binding: Low Affinity Site kon (nM−1 s−1)	Biacore Binding: Low Affinity Site koff (s−1)
0.0016	0.0044	0.00061	0.13
(1.1)	(1.1)	(1.3)	(1.3)