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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Mol Neurobiol. 2017 Oct 5;55(7):5611–5622. doi: 10.1007/s12035-017-0781-2

Figure 1. F30 (AZI23′UTR) allele-dependent downregulation promoters activity by luciferase reporting in the human neuroblastoma SK-N-AS cells and downregulation of endogenous SLC6A3 mRNA level in the human neuroblastoma BE(2)-M17 cells.

Figure 1

(a) SV40 promoter or (b) on 7.9kb SLC6A3 promoter, based on effects of F30 vs. vector pcDNA3.1 overexpression. A and B represent promoter carrying DNPiA or DNPiB alleles, respectively. Indicated P-value was obtained from Student’s t-tests; ANOVA P = 0.0028 for F30 effects on both SV40 and 7.9kb SLC6A3 promoters, compared to vector activity (n = 7–10); (c) F30 DsiRNA exerts enhancing effects on the SV40-B promoter but not the SV40-A promoter (d), based on one-way ANOVA (n = 8–12). Three different F30 DsiRNA molecules were used (sequences listed in Supplementary Table 1). The SV40 data in panel (a) and DsiRNA effects in panels (c) and (d) were replicated in HEK293T and SH-SY5Y cells (see Supplementary Figs. 1 and 2).(e) different doses pcDNA3.1-F30 vector and (f) pcDNA3.1 vector. One-way ANOVA: *, P < 0.05; **, P < 0.001; ***, P < 0.0001 (n = 3–6).