Skip to main content
. 2016 Apr 7;2:70–77. doi: 10.1016/j.pvr.2016.04.001

Fig. 4.

Fig. 4.

Loss of hScrib decreases the levels of HPV-18 E6 mRNA. Panel A. HeLa cells were transfected with siRNA against Luciferase or siRNA HPV-18 E6/E7 or siRNA hScrib. 72 h after transfection, cells were harvested and the expression patterns of: pRB as a surrogate marker for E7 expression, p53 to monitor E6/E7 loss, hScrib and α-actinin, to monitor the protein loading, were assessed by western blot. Panel B. Band intensities of hypo-phosphorylated and hyper-phosphorylated pRB were determined using the OptiQuant quantification program. Levels of pRB expression are expressed as the % of hyper- and hypo-phosphorylated pRB relative to siLuciferase-transfected control cells. Standard deviations are also shown. Panel D. The graph shows the result of quantitative real time pcr for the levels of E6 mRNA, using GAPDH as the control. The bars show the relative levels of E6 expression where siLuc is used as the reference. Note the greater than 60% reduction in E6 mRNA following transfection with either siE6/E7 or siScrib. The numbers represent the means from 3 independent experiments and standard deviations are shown. Panel C shows the accompanying western blot verifying ablation of hScrib expression in a representative assay.