Fig. 5.

hScrib regulates the translation of HPV-18 E6 in HeLa cells. Panel A. HeLa cells were transfected with siRNA against Luciferase or siRNA against hScrib. 72 h after transfection, cells were treated with cycloheximide for 6 h. Prior to harvesting the cells were washed three times with PBS to remove the cycloheximide and protein translation was left to recover in complete medium for 4 different time points: 0.5, 1, 3 and 5 h. The collated results from 3 independent experiments are shown in panel B. Band intensities were determined using the OptiQuant quantification program. The E6 levels in siLuciferase and siScrib transfected cells were normalized to 100% at time 0. Standard deviations are also shown.