Figure 6.

Light micrographs illustrating in situ hybridization of DIG-labeled PsAAP1 antisense (A and B) and sense (C) riboprobes to transverse sections of developing pea cotyledons. Probe binding in the cytoplasm of the epidermal transfer cells and the storage parenchyma cells is evident as a strong blue color indicated by darts in B (compare with the sense control, C). The dense color reactant in the epidermal transfer cells and five to six rows of storage parenchyma cells inward of the epidermis declines through subsequent consecutive rows of storage parenchyma cells (A). In A, the bar = 100 μm (×120); in B and C, the bar = 30 μm (×300). cw, Cell wall; et, epidermal transfer cell; sg, starch granule; sp, storage parenchyma cell; and v, vacuole.