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. 2018 Mar 22;12(2):101–109. doi: 10.4162/nrp.2018.12.2.101

Fig. 4. The effects of vitamin C on colon tumor number and mRNA expression of PCNA.

Fig. 4

(A) Number of colon tumor were counted with naked eye when sacrificing the mice. (B) mRNA expressions of PCNA was analyzed in the middle colon (a) and in the distal colon (b) using real-time PCR. β-actin and GAPDH were used as the loading control. Mean ± SEM are shown (Control, n = 9; CC, n = 12; V60 and V120, n = 11 per group). One-way ANOVAs and Newman-Keuls' post hoc tests were performed (P<0.05). CC, AOM/DSS-induced colon cancer; V60, AOM/DSS + 60 mg/kg body weight (b.w.) of vitamin C; V120, AOM/DSS + 120 mg/kg b.w. of vitamin C.